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KMID : 1101720190230020001
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Physical Activity and Nutrition
2019 Volume.23 No. 2 p.1 ~ p.6
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Acid sphingomyelinase inhibition alleviates muscle damage in gastrocnemius after acute strenuous exercise
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Lee Young-Ik
Leem Yea-Hyun
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Abstract
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Purpose: Strenuous exercise often induces skeletal muscle damage, which results in impaired performance. Sphingolipid metabolism contributes to various cellular processes, including apoptosis, stress response, and inflammation. However, the relationship between exercise-induced muscle damage and ceramide (a key component of sphingolipid metabolism), is rarely studied. The present study aimed to explore the regulatory role of sphingolipid metabolism in exercise-induced muscle damage.
Methods: Mice were subjected to strenuous exercise by treadmill running with gradual increase in intensity. The blood and gastrocnemius muscles (white and red portion) were collected immediately after and 24 h post exercise. For 3 days, imipramine was intraperitoneally injected 1 h prior to treadmill running.
Results: Interleukin 6 (IL-6) and serum creatine kinase (CK) levels were enhanced immediately after and 24 h post exercise (relative to those of resting), respectively. Acidic sphingomyelinase (A-SMase) protein expression in gastrocnemius muscles was significantly augmented by exercise, unlike, serine palmitoyltransferase-1 (SPT-1) and neutral sphingomyelinase (N-SMase) expressions. Furthermore, imipramine (a selective A-SMase inhibitor) treatment reduced the exercise-induced CK and IL-6 elevations, along with a decrease in cleaved caspase-3 (Cas-3) of gastrocnemius muscles.
Conclusion: We found the crucial role of A-SMase in exercise-induced muscle damage.
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KEYWORD
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Strenuous exercise, muscle damage, creatine kinase, interleukin-6, sphingomyelinase, imipramine
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